product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PHGDH Antibody (clone 4A3-1D6) LS-B5457
catalog :
LS-B5457
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4A3-1D6
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human
Gromova I, Gromov P, Honma N, Kumar S, Rimm D, Talman M, et al. High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy. Mol Oncol. 2015;9:1636-54 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5457 image 1
Western blot of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody, clone 4A3-1D6.
image 2 :
LifeSpan Biosciences LS-B5457 image 2
Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1 ~10 ug/ml].
image 3 :
LifeSpan Biosciences LS-B5457 image 3
Immunoprecipitation of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead.
product information
AntibodyID :
140006
AntibodyName :
LS-B5457
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ PHGDH Antibody (clone 4A3-1D6) LS-B5457
Specificity :
Human PHGDH
ClonalityDesc :
Monoclonal
CloneName :
4A3-1D6
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
PHGDH (AAH11262, 1 aa ~ 534 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
PurificationDesc :
Purified from ascites by Protein A
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
PHGDH
StandardGeneSymbol :
PHGDH
Reactivity :
Human
Usage :
ELISA, IF (1 - 10 µg/ml), IHC, IHC-P (5 µg/ml), IP, WB
ShortWebDescription :
PHGDH antibody LS-B5457 is an unconjugated mouse monoclonal antibody to human PHGDH. Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.
Synonyms :
PHGDH, 3-PGDH, 3PGDH, PGDH, Phosphoglycerate dehydrogenase, PDG, PGAD, PGDH3
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.