product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PDCD1 / CD279 / PD-1 Antibody LS-B540
catalog :
LS-B540
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Ji D, Yi H, Zhang D, Zhan T, Li Z, Li M, et al. Somatic Mutations and Immune Alternation in Rectal Cancer Following Neoadjuvant Chemoradiotherapy. Cancer Immunol Res. 2018;6:1401-1416 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B540 image 1
Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
image 2 :
LifeSpan Biosciences LS-B540 image 2
Immunofluorescence of PD-1 in Human Brain cells with PD-1 antibody at 20 ug/ml.
image 3 :
LifeSpan Biosciences LS-B540 image 3
Western blot of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
product information
AntibodyID :
32768
AntibodyName :
LS-B540
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ PDCD1 / CD279 / PD-1 Antibody LS-B540
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS containing 0.02% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A 16 AA synthetic peptide near the C-Terminus of human PD-1. The immunogen is located within aa 210-260 of PD-1.
PurificationDesc :
Peptide Column Affinity Chromatography
RecommendedStorageDesc :
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
PDCD1 / CD279 / PD-1
BlockingPeptide :
LS-E30321
StandardGeneSymbol :
PDCD1
gene family :
Apoptosis
Reactivity :
Mouse, Human
Usage :
ELISA, IF, IHC, IHC-P (2.5 µg/ml), WB
ShortWebDescription :
PD-1 antibody LS-B540 is an unconjugated rabbit polyclonal antibody to PD-1 (PDCD1 / CD279) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 45 kDa
Synonyms :
PDCD1, CD279, HPD-1, HPD-l, PD1, Protein PD-1, SLEB2, PD-1, CD279 antigen, Programmed cell death 1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.