This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MHC Class II RT-1B Antibody (clone OX-6) LS-B5258
catalog :
LS-B5258
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
OX-6

The same clone is also sold as:
reactivity :
rat
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
139807
AntibodyName :
LS-B5258
TargetSpecies :
Rat
Host Species :
Mouse
Product Name :
IHC-plus™ MHC Class II RT-1B Antibody (clone OX-6) LS-B5258
Specificity :
Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.
ClonalityDesc :
Monoclonal
CloneName :
OX-6
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Membrane extract
ImmunogenDesc :
Rat thymocyte membrane glycoprotein.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C.
IsotypeName :
IgG1
Gene :
MHC Class II RT-1B
Reactivity :
Rat
Usage :
Flo (1:50 - 1:100), IF, IHC, IHC-Fr, IHC-P (10 µg/ml)
ShortWebDescription :
MHC Class II RT-1B antibody LS-B5258 is an unconjugated mouse monoclonal antibody to rat MHC Class II RT-1B. Validated for Flow, IF and IHC.
UsageText :
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.