product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ NES / Nestin Antibody LS-B5224
catalog :
LS-B5224
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Mirzadeh Z, Kusne Y, Duran Moreno M, Cabrales E, Gil Perotin S, Ortiz C, et al. Bi- and uniciliated ependymal cells define continuous floor-plate-derived tanycytic territories. Nat Commun. 2017;8:13759 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5224 image 1
Anti-Nestin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
139773
AntibodyName :
LS-B5224
TargetSpecies :
Human
Host Species :
Chicken
Product Name :
PathPlus™ NES / Nestin Antibody LS-B5224
Specificity :
Recognizes human and mouse Nestin. Immunogenic peptide has 100 % homology between rat and human but the antibody has not been tested with rat samples.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.2, 0.02% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Peptide corresponded to a region of the Nestin gene product shared between the rat (AAA41119, NCBI) and human (NP_001966, NCBI) gene products.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
IsotypeName :
IgY
Gene :
NES / Nestin
StandardGeneSymbol :
NES
Reactivity :
Mouse, Human
Usage :
ICC (1:50 - 1:100), IHC, IHC-P (5 µg/ml), WB (1:125 - 1:250)
ShortWebDescription :
Nestin antibody LS-B5224 is an unconjugated chicken polyclonal antibody to Nestin (NES) from human. It is reactive with human and mouse. Validated for ICC, IHC and WB. Cited in 1 publication.
UsageText :
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:125-1:250. Immunohistochemistry: 1:50-1:100. Immunocytochemistry: 1:50-1:100.
Synonyms :
NES, Nestin, Nbla00170
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.