product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ HIF2A / EPAS1 Antibody (aa535-631, clone ep190b) LS-B501
catalog :
LS-B501
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ep190b
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; human
Lane R, Fu Y, Matsuzaki S, Kinter M, Humphries K, Griffin T. Mitochondrial respiration and redox coupling in articular chondrocytes. Arthritis Res Ther. 2015;17:54 pubmed publisher
Okamoto N, Tanaka A, Jung K, Karasawa K, Orito K, Matsuda A, et al. Silencing of int6 gene restores function of the ischaemic hindlimb in a rat model of peripheral arterial disease. Cardiovasc Res. 2011;92:209-17 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B501 image 1
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
31460
AntibodyName :
LS-B501
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ HIF2A / EPAS1 Antibody (aa535-631, clone ep190b) LS-B501
Specificity :
HIF-2 alpha and does not cross-react with HIF-1 alpha.
ClonalityDesc :
Monoclonal
CloneName :
ep190b
AntibodyModification :
Unconjugated
AntigenModification :
aa535-631
PresentationDesc :
PBS, 0.05% Sodium Azide, 1% BSA
ImmunogenDesc :
Human HIF-2 alpha amino acids 535-631
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
HIF2A / EPAS1
StandardGeneSymbol :
EPAS1
Reactivity :
Mouse, Rat, Human
Usage :
IHC, IHC-P (10 - 20 µg/ml), WB
ShortWebDescription :
EPAS1 antibody LS-B501 is an unconjugated mouse monoclonal antibody to EPAS1 (HIF2A) (aa535-631) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B501 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B501 was determined to be 10-20 ug/ml.
Synonyms :
EPAS1, BHLHe73, ECYT4, HIF-1-alpha-like factor, HIF2-alpha, HIF2A, EPAS-1, HIF-2-alpha, HIF-1alpha-like factor, MOP2, Member of PAS protein 2, PASD2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.