product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ KITLG / SCF Antibody LS-B5002
catalog :
LS-B5002
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human
  • western blot; human
Fukuzawa R, Anaka M, Morison I, Reeve A. The developmental programme for genesis of the entire kidney is recapitulated in Wilms tumour. PLoS ONE. 2017;12:e0186333 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5002 image 1
Western blot of SCF in rat brain tissue lysate with SCF antibody at (A) 1 and (B) 2 ug/ml.
image 2 :
LifeSpan Biosciences LS-B5002 image 2
Anti-KITLG / SCF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
image 3 :
LifeSpan Biosciences LS-B5002 image 3
Immunofluorescence of SCF in Human Brain cells with SCF antibody at 20 ug/ml.
product information
AntibodyID :
128412
AntibodyName :
LS-B5002
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ KITLG / SCF Antibody LS-B5002
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS containing 0.02% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
An 18 AA synthetic peptide near the center of human SCF. The immunogen is located within aa 100-150 of SCF.
PurificationDesc :
Peptide Column Affinity Chromatography
RecommendedStorageDesc :
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
KITLG / SCF
BlockingPeptide :
LS-E30702
StandardGeneSymbol :
KITLG
Reactivity :
Mouse, Rat, Human
Usage :
ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
ShortWebDescription :
SCF antibody LS-B5002 is an unconjugated rabbit polyclonal antibody to SCF (KITLG) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
SCF antibody can be used for detection of SCF by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 24,30 kDa Observed: 23, 28 kDa
Synonyms :
KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7, SF, Steel factor, Stem cell factor, SCF, FPH2, Mast cell growth factor, MGF
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.