product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa530-825) LS-B495
catalog :
LS-B495
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Reference
Qureshi Baig K, Kuhn D, Viry E, Pozdeev V, Schmitz M, Rodriguez F, et al. Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway. Autophagy. 2020;16:1436-1452 pubmed publisher
Kron P, Linecker M, Limani P, Schlegel A, Kambakamba P, Lehn J, et al. Hypoxia-driven Hif2a coordinates mouse liver regeneration by coupling parenchymal growth to vascular expansion. Hepatology. 2016;64:2198-2209 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B495 image 1
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
31454
AntibodyName :
LS-B495
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa530-825) LS-B495
Specificity :
HIF-1 alpha.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa530-825
PresentationDesc :
PBS, 0.02% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
A fusion protein including aa 530-825 of the mouse HIF-1 alpha protein.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
HIF1A / HIF1 Alpha
StandardGeneSymbol :
HIF1A
Reactivity :
Rabbit, Mouse, Goat, Rat, Hamster, Primate, Human
Usage :
IHC, IHC-P (5 - 10 µg/ml)
ShortWebDescription :
HIF1 Alpha antibody LS-B495 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa530-825) from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC. Cited in 6 publications.
UsageText :
Immunohistochemistry: LS-B495 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B495 was determined to be 5-10 ug/ml.
Synonyms :
HIF1A, ARNT-interacting protein, ARNT interacting protein, BHLHe78, HIF1 Alpha, Hypoxia-inducible factor1alpha, HIF-1A, HIF1, MOP1, Member of PAS superfamily 1, PASD8, HIF-1-alpha, HIF-1alpha, HIF1-ALPHA, Member of PAS protein 1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.