IHC-plus™ UCHL1 / PGP9.5 Antibody (aa100-200) LS-B489 | LifeSpan Biosciences LS-B489 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ UCHL1 / PGP9.5 Antibody (aa100-200) LS-B489

catalog :

LS-B489

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, domestic sheep

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; domestic sheep; 3 ug/ml; fig s4 western blot; human	Lea R, Amezaga M, Loup B, Mandon PÃ©pin B, Stefansdottir A, Filis P, et al. The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals. Sci Rep. 2016;6:22279 pubmed publisher

image

image 1 :

Anti-UCHL1 / PGP9.5 antibody IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

product information

AntibodyID :

31448

AntibodyName :

LS-B489

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

IHC-plus™ UCHL1 / PGP9.5 Antibody (aa100-200) LS-B489

Specificity :

Rabbit Polyclonal to PGP9.5 - Peripheral Nerve Fiber marker.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa100-200

PresentationDesc :

Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

A synthetic peptide made to an internal portion of the human UCHL1 protein sequence (between residues 100-200). UniProt P09936. Percent identity by BLAST analysis: Primate, Guinea (100%); Rat (99%); Bovine (92%).

PurificationDesc :

Immunoaffinity purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Gene :

UCHL1 / PGP9.5

StandardGeneSymbol :

UCHL1

gene family :

Protease

Subfamily :

Cysteine C12

Reactivity :

Human

Usage :

IHC, IHC-P (2.5 - 5 µg/ml), WB (0.5 - 2 µg/ml)

ShortWebDescription :

PGP9.5 antibody LS-B489 is an unconjugated rabbit polyclonal antibody to human PGP9.5 (UCHL1) (aa100-200). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B489 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B489 was determined to be 2.5-5 ug/ml.

Synonyms :

UCHL1, Ubiquitin C-terminal hydrolase, PARK5, PGP9.5, PGP95, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Uch-L1, Ubiquitin carboxyl-terminal esterase L1, HEL-117, NDGOA, HEL-S-53, SPG79

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.