product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ IREB2 / IRP2 Antibody (aa100-200) LS-B48
catalog :
LS-B48
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 1
  • immunocytochemistry; mouse
Cloonan S, Glass K, Laucho Contreras M, Bhashyam A, Cervo M, Pabón M, et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med. 2016;22:163-74 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B48 image 1
Anti-IREB2 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
27088
AntibodyName :
LS-B48
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
IHC-plus™ IREB2 / IRP2 Antibody (aa100-200) LS-B48
Specificity :
Rabbit Polyclonal to IRP2.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa100-200
PresentationDesc :
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). UniProt Q811J3. Percent identity by BLAST analysis: Human, Primate, Rat (94%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Gene :
IREB2 / IRP2
StandardGeneSymbol :
IREB2
Reactivity :
Mouse, Human
Usage :
ICC (1:500), IHC, IHC-P (20 µg/ml), WB (2 µg/ml)
ShortWebDescription :
IRP2 antibody LS-B48 is an unconjugated rabbit polyclonal antibody to IRP2 (IREB2) (aa100-200) from mouse. It is reactive with human and mouse. Validated for ICC, IHC and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B48 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B48 was determined to be 20 ug/ml.
Synonyms :
IREB2, ACO3, Iron regulatory protein 2, IRP2AD, IRE-BP 2, IRP2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.