product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TGF-Beta Antibody (clone TB21) LS-B4772
catalog :
LS-B4772
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
TB21
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, neutralization, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human
Donatelli S, Zhou J, Gilvary D, Eksioglu E, Chen X, Cress W, et al. TGF-?-inducible microRNA-183 silences tumor-associated natural killer cells. Proc Natl Acad Sci U S A. 2014;111:4203-8 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B4772 image 1
Anti-TGF-Beta antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
124465
AntibodyName :
LS-B4772
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ TGF-Beta Antibody (clone TB21) LS-B4772
Specificity :
Recognizes both human platelet-derived and recombinant TGF-beta.
ClonalityDesc :
Monoclonal
CloneName :
TB21
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.0
ImmunogenType :
Purified protein
ImmunogenDesc :
TGF-b from human platelets.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
TGF-Beta
Reactivity :
Human
Usage :
ELISA, Func, IHC, IHC-Fr, IHC-P (10 µg/ml), Neut, WB
ShortWebDescription :
TGF-Beta antibody LS-B4772 is an unconjugated mouse monoclonal antibody to human TGF-Beta. Validated for ELISA, Func, IHC, Neut and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Suitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Neutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry (formalin-fixed, paraffin-embedded, frozen): 1:1000. Has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blot: 5-20 ng/ml will allow visualization of 100 ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively.
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.