product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SQSTM1 Antibody LS-B4617
catalog :
LS-B4617
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse
Hubbs A, Fluharty K, Edwards R, Barnabei J, Grantham J, Palmer S, et al. Accumulation of Ubiquitin and Sequestosome-1 Implicate Protein Damage in Diacetyl-Induced Cytotoxicity. Am J Pathol. 2016;186:2887-2908 pubmed publisher
Xu S, Lun Y, Frascella M, Garcia A, Soska R, Nair A, et al. Improved efficacy of a next-generation ERT in murine Pompe disease. JCI Insight. 2019;4: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B4617 image 1
Western blot of SQSTM1 in Human spleen tissue lysate with SQSTM1 antibody at (A) 1 and (B) 2 ug/ml.
image 2 :
LifeSpan Biosciences LS-B4617 image 2
Anti-SQSTM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
image 3 :
LifeSpan Biosciences LS-B4617 image 3
Immunofluorescence of SQSTM1 in Rat Spleen cells with SQSTM1 antibody at 20 ug/ml.
product information
AntibodyID :
124283
AntibodyName :
LS-B4617
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SQSTM1 Antibody LS-B4617
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS containing 0.02% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A 14 AA synthetic peptide near the C-Terminus of human SQSTM1. The immunogen is located within the last 50 AA of SQSTM1.
PurificationDesc :
Peptide Column Affinity Chromatography
RecommendedStorageDesc :
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
SQSTM1
BlockingPeptide :
LS-E30797
StandardGeneSymbol :
SQSTM1
Reactivity :
Mouse, Rat, Human
Usage :
ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
ShortWebDescription :
SQSTM1 antibody LS-B4617 is an unconjugated rabbit polyclonal antibody to SQSTM1 from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
SQSTM1 antibody can be used for detection of SQSTM1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested. Western Blot:
Synonyms :
SQSTM1, A170, ORCA, OSIL, Paget disease of bone 3, p60, p62, PDB3, Sequestosome-1, Ubiquitin-binding protein p62, Oxidative stress induced like, p62B, ZIP3, Sequestosome 1, EBI3-associated protein p60, EBIAP
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.