This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PPARA / PPAR Alpha Antibody (aa1-25) LS-B46
catalog :
LS-B46
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
product information
AntibodyID :
27086
AntibodyName :
LS-B46
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
IHC-plus™ PPARA / PPAR Alpha Antibody (aa1-25) LS-B46
Specificity :
A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1-25
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Expiration date is one year from opening.
IsotypeName :
IgG
Gene :
PPARA / PPAR Alpha
StandardGeneSymbol :
PPARA
gene family :
NHR
Subfamily :
NR1 Thyroid hormone-like
Reactivity :
Mouse, Dog, Bovine, Rat, Hamster-Golden Syrian, Boar
Usage :
ELISA (1:75000 - 1:125000), Flo, IF (1 - 5 µg/ml), IHC, IHC-P (20 µg/ml), WB (1:500 - 1:2000)
ShortWebDescription :
PPAR Alpha antibody LS-B46 is an unconjugated rabbit polyclonal antibody to PPAR Alpha (PPARA) (aa1-25) from mouse. It is reactive with mouse, rat, boar and other species. Validated for ELISA, Flow, IF, IHC and WB.
UsageText :
Immunohistochemistry: LS-B46 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B46 was determined to be 20 ug/ml.
Synonyms :
PPARA, PPAR Alpha, PPARalpha, PPAR-alpha, NR1C1, HPPAR, PPAR
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.