IHC-plus™ WNT5A Antibody (aa180-229) LS-B4565 | LifeSpan Biosciences LS-B4565 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ WNT5A Antibody (aa180-229) LS-B4565

catalog :

LS-B4565

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human	Newman D, Sills W, Hanrahan K, Ziegler A, Tidd K, Cook E, et al. Expression of WNT5A in Idiopathic Pulmonary Fibrosis and Its Control by TGF-Î² and WNT7B in Human Lung Fibroblasts. J Histochem Cytochem. 2016;64:99-111 pubmed publisher
	Wilson D, Jarman E, Mellin R, Wilson M, Waddell S, Tsokkou P, et al. Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors. Nat Commun. 2020;11:445 pubmed publisher
	Wu X, Hu L, Li Y, Li Y, Wang F, Ma P, et al. SCAPs Regulate Differentiation of DFSCs During Tooth Root Development in Swine. Int J Med Sci. 2018;15:291-299 pubmed publisher

image

image 1 :

Anti-WNT5A antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

product information

AntibodyID :

120410

AntibodyName :

LS-B4565

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

IHC-plus™ WNT5A Antibody (aa180-229) LS-B4565

Specificity :

Human WNT5A

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa180-229

PresentationDesc :

PBS, 0.09% sodium azide, 2% sucrose

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

Synthetic peptide located between aa180-229 of human WNT5A (P41221, NP_003383). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Pig, Opossum, Guinea pig, Platypus, Lizard (100%); Monkey, Zebra finch, Chicken, Xenopus (93%); Orangutan, Hamster, Horse, Turkey (86%); Panda (81%); Stickleback, Zebrafish (80%).

PurificationDesc :

Immunoaffinity purified

RecommendedStorageDesc :

Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

IsotypeName :

IgG

Gene :

WNT5A

BlockingPeptide :

LS-E11228

StandardGeneSymbol :

WNT5A

gene family :

WNT

Reactivity :

Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Bat, Human

Usage :

IHC, IHC-P (5 µg/ml), WB

ShortWebDescription :

WNT5A antibody LS-B4565 is an unconjugated rabbit polyclonal antibody to WNT5A (aa180-229) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.

UsageText :

ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Synonyms :

WNT5A, HWNT5A, Protein Wnt-5a, WNT-5A protein

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.