product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SIRT1 / Sirtuin 1 Antibody (clone 7B7) LS-B4520
catalog :
LS-B4520
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7B7
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Chen H, Wang F, Gao P, Pei J, Liu Y, Xu T, et al. Age-Associated Sirtuin 1 Reduction in Vascular Smooth Muscle Links Vascular Senescence and Inflammation to Abdominal Aortic Aneurysm. Circ Res. 2016;119:1076-1088 pubmed
image
image 1 :
LifeSpan Biosciences LS-B4520 image 1
SIRT1 monoclonal antibody, clone 7B7 Western blot of SIRT1 expression in HeLa NE.
image 2 :
LifeSpan Biosciences LS-B4520 image 2
Immunofluorescence of monoclonal antibody to SIRT1 on HeLa cell (antibody concentration 10 ug/ml).
image 3 :
LifeSpan Biosciences LS-B4520 image 3
Detection limit for recombinant GST tagged SIRT1 is approximately 0.1 ng/ml as a capture antibody.
product information
AntibodyID :
120364
AntibodyName :
LS-B4520
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ SIRT1 / Sirtuin 1 Antibody (clone 7B7) LS-B4520
ClonalityDesc :
Monoclonal
CloneName :
7B7
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
SIRT1 (AAH12499, 456 aa ~ 555 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
PurificationDesc :
Purified from ascites by Protein A
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
SIRT1 / Sirtuin 1
StandardGeneSymbol :
SIRT1
gene family :
ADP-Ribosyltransferase
Reactivity :
Human
Usage :
ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB
ShortWebDescription :
Sirtuin 1 antibody LS-B4520 is an unconjugated mouse monoclonal antibody to human Sirtuin 1 (SIRT1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Western Blot (Cell lysate) - positive control Hela NE. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Synonyms :
SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.