IHC-plus™ ADAM17 / TACE Antibody (clone 1F6) LS-B4426 | LifeSpan Biosciences LS-B4426 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ ADAM17 / TACE Antibody (clone 1F6) LS-B4426

catalog :

LS-B4426

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F6

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, proximity ligation assay

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Orme J, Jazieh K, Xie T, Harrington S, Liu X, Ball M, et al. ADAM10 and ADAM17 cleave PD-L1 to mediate PD-(L)1 inhibitor resistance. Oncoimmunology. 2020;9:1744980 pubmed publisher

image

image 1 :

Proximity Ligation Analysis (PLA) of protein-protein interactions between TGFA and ADAM17. HeLa cells were stained with anti-TGFA rabbit purified polyclonal 1:1200 and anti-ADAM17 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

image 2 :

Detection limit for recombinant GST tagged ADAM17 is approximately 3 ng/ml as a capture antibody.

image 3 :

Anti-ADAM17 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

AntibodyID :

120263

AntibodyName :

LS-B4426

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ ADAM17 / TACE Antibody (clone 1F6) LS-B4426

ClonalityDesc :

Monoclonal

CloneName :

1F6

AntibodyModification :

Unconjugated

PresentationDesc :

PBS, pH 7.4

ImmunogenType :

Recombinant protein

ImmunogenDesc :

ADAM17 (NP_003174, 215 aa ~ 315 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

PurificationDesc :

Purified from ascites by Protein A

RecommendedStorageDesc :

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

IsotypeName :

IgG2b,k

Gene :

ADAM17 / TACE

StandardGeneSymbol :

ADAM17

gene family :

Protease

Subfamily :

Metallopeptidase M12B

Reactivity :

Human

Usage :

ELISA, IHC, IHC-P (5 µg/ml), PLA, WB (1:500 - 1:1000)

ShortWebDescription :

TACE antibody LS-B4426 is an unconjugated mouse monoclonal antibody to human TACE (ADAM17). Validated for ELISA, IHC, PLA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Western blot performed with the recombinant protein used as the immunogen. In situ Proximity Ligation Assay (Cell).

Synonyms :

ADAM17, CD156B, CD156b antigen, CSVP, ADAM 17, NISBD, TNF-alpha converting enzyme, TNF-alpha convertase, TNF-alpha-converting enzyme, Snake venom-like protease, TACE

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.