product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MBNL1 / MBNL Antibody (clone 3E7) LS-B4372
catalog :
LS-B4372
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3E7
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 3
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 5c
Wongpalee S, Vashisht A, Sharma S, Chui D, Wohlschlegel J, Black D. Large-scale remodeling of a repressed exon ribonucleoprotein to an exon definition complex active for splicing. elife. 2016;5: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s3a
Chaudhury A, Cheema S, Fachini J, Kongchan N, Lu G, Simon L, et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. Nat Commun. 2016;7:13362 pubmed publisher
  • western blot; human; fig 1a
Giudice J, Xia Z, Li W, Cooper T. Neonatal cardiac dysfunction and transcriptome changes caused by the absence of Celf1. Sci Rep. 2016;6:35550 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B4372 image 1
MBNL1 monoclonal antibody clone 3E7 Western blot of MBNL1 expression in HeLa NE.
image 2 :
LifeSpan Biosciences LS-B4372 image 2
Immunofluorescence of monoclonal antibody to MBNL1 on HeLa cell (antibody concentration 10 ug/ml).
image 3 :
LifeSpan Biosciences LS-B4372 image 3
Detection limit for recombinant GST tagged MBNL1 is approximately 3 ng/ml as a capture antibody.
product information
AntibodyID :
120209
AntibodyName :
LS-B4372
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ MBNL1 / MBNL Antibody (clone 3E7) LS-B4372
ClonalityDesc :
Monoclonal
CloneName :
3E7
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
MBNL1 (AAH43493, 1 aa ~ 383 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
PurificationDesc :
Purified from ascites by Protein A
RecommendedStorageDesc :
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
IsotypeName :
IgG2a,k
Gene :
MBNL1 / MBNL
StandardGeneSymbol :
MBNL1
Reactivity :
Human
Usage :
ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB
ShortWebDescription :
MBNL antibody LS-B4372 is an unconjugated mouse monoclonal antibody to human MBNL (MBNL1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Western Blot (Cell lysate) - positive control Hela NE. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Synonyms :
MBNL1, EXP35, EXP40, KIAA0428, Muscleblind (Drosophila)-like, Muscleblind-like protein 1, MBNL, EXP, EXP42, Muscleblind-like (Drosophila)
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.