PathPlus™ NALP3 / NLRP3 Antibody LS-B4321 | LifeSpan Biosciences LS-B4321 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

PathPlus™ NALP3 / NLRP3 Antibody LS-B4321

catalog :

LS-B4321

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; rat	Coucha M, Mohamed I, Elshaer S, Mbata O, Bartasis M, El Remessy A. High fat diet dysregulates microRNA-17-5p and triggers retinal inflammation: Role of endoplasmic-reticulum-stress. World J Diabetes. 2017;8:56-65 pubmed publisher
	Mohamed I, Sheibani N, El Remessy A. Deletion of Thioredoxin-Interacting Protein (TXNIP) Abrogates High Fat Diet-induced Retinal Leukostasis, Barrier Dysfunction and Microvascular Degeneration in a Mouse Obesity Model. Int J Mol Sci. 2020;21: pubmed publisher
	Andras I, Garcia Contreras M, Yanick C, Perez P, Sewell B, Durand L, et al. Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection. Mol Brain. 2020;13:21 pubmed publisher
	Coucha M, Shanab A, Sayed M, Vazdarjanova A, El Remessy A. Modulating Expression of Thioredoxin Interacting Protein (TXNIP) Prevents Secondary Damage and Preserves Visual Function in a Mouse Model of Ischemia/Reperfusion. Int J Mol Sci. 2019;20: pubmed publisher
	Tavakoli Dargani Z, Singla D. Embryonic stem cell-derived exosomes inhibit doxorubicin-induced TLR4-NLRP3-mediated cell death-pyroptosis. Am J Physiol Heart Circ Physiol. 2019;317:H460-H471 pubmed publisher
	Elshaer S, Mohamed I, Coucha M, Altantawi S, Eldahshan W, Bartasi M, et al. Deletion of TXNIP Mitigates High-Fat Diet-Impaired Angiogenesis and Prevents Inflammation in a Mouse Model of Critical Limb Ischemia. Antioxidants (Basel). 2017;6: pubmed publisher

image

image 1 :

Western blot of NLRP3 in K562 cell lysate with NLRP3 antibody at 1 ug/ml (A) in the absence and (B) in the presence of blocking peptide.

image 2 :

Anti-NLRP3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

product information

AntibodyID :

120158

AntibodyName :

LS-B4321

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

PathPlus™ NALP3 / NLRP3 Antibody LS-B4321

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

PresentationDesc :

PBS containing 0.02% Sodium Azide

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

A 16 AA synthetic peptide near the N-Terminus of human NALP3. The immunogen is located within aa 120-170 of NALP3.

PurificationDesc :

Peptide Column Affinity Chromatography

RecommendedStorageDesc :

Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

IsotypeName :

IgG

Gene :

NALP3 / NLRP3

BlockingPeptide :

LS-E30796

StandardGeneSymbol :

NLRP3

Reactivity :

Mouse, Human

Usage :

ELISA, ICC (2 µg/ml), IF (20 µg/ml), IHC, IHC-P (2.5 - 5 µg/ml), WB (1 µg/ml)

ShortWebDescription :

NLRP3 antibody LS-B4321 is an unconjugated rabbit polyclonal antibody to NLRP3 (NALP3) from human. It is reactive with human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Cited in 7 publications.

UsageText :

NALP3 antibody can be used for detection of NALP3 by Western blot at 1 ug/mL. Antibody can also be used for Immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 101, 114 kDa Observed: 110 kDa

Synonyms :

NLRP3, AGTAVPRL, AII, C1orf7, Caterpiller protein 1.1, Cryopyrin, FCAS, FCU, PYPAF1, NALP3, CIAS1, CLR1.1, MWS

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage