product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TP53 / p53 Antibody (clone BP53-12) LS-B431
catalog :
LS-B431
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BP53-12
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 3
Reference
Gurney E, Harrison R, Fenno J. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980;34:752-63 pubmed
Hollstein M, Sidransky D, Vogelstein B, Harris C. p53 mutations in human cancers. Science. 1991;253:49-53 pubmed
Lane D. Cancer. p53, guardian of the genome. Nature. 1992;358:15-6 pubmed
image
image 1 :
LifeSpan Biosciences LS-B431 image 1
Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
31390
AntibodyName :
LS-B431
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ TP53 / p53 Antibody (clone BP53-12) LS-B431
Specificity :
Reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.
ClonalityDesc :
Monoclonal
CloneName :
BP53-12
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a,k
Gene :
TP53 / p53
StandardGeneSymbol :
TP53
gene family :
P53
Reactivity :
Human
Usage :
ChrIP (1 µg/µl), ELISA (1:2000 - 1:10000), IF (1:100 - 1:500), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:500 - 1:2000)
ShortWebDescription :
P53 antibody LS-B431 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for ChrIP, ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B431 was determined to be 2.5 ug/ml.
Synonyms :
TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.