product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NRF1 / NRF-1 Antibody LS-B43
catalog :
LS-B43
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 3a
Zou P, Liu L, Zheng L, Payne K, Manjili M, Idowu M, et al. Coordinated Upregulation of Mitochondrial Biogenesis and Autophagy in Breast Cancer Cells: The Role of Dynamin Related Protein-1 and Implication for Breast Cancer Treatment. Oxid Med Cell Longev. 2016;2016:4085727 pubmed
image
image 1 :
LifeSpan Biosciences LS-B43 image 1
Anti-NRF1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
27083
AntibodyName :
LS-B43
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
IHC-plus™ NRF1 / NRF-1 Antibody LS-B43
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1- 534 of the native protein.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
NRF1 / NRF-1
StandardGeneSymbol :
NRF1
gene family :
Transcription factor
Reactivity :
Mouse
Usage :
ELISA (1:3000 - 1:10000), IHC, IHC-P (2 - 5 µg/ml), WB (1:500 - 1:2000)
ShortWebDescription :
NRF-1 antibody LS-B43 is an unconjugated rabbit polyclonal antibody to mouse NRF-1 (NRF1). Validated for ELISA, IHC and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B43 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B43 was determined to be 2-5 ug/ml.
Synonyms :
NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.