This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TFAP2A / AP-2 Antibody (aa1-25) LS-B427
catalog :
LS-B427
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
31386
AntibodyName :
LS-B427
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ TFAP2A / AP-2 Antibody (aa1-25) LS-B427
Specificity :
A BLAST analysis was used to suggest reactivity with AP2A1 and AP2A2 protein from human, chimpanzee, orangutan, mouse, rat, chicken and dog based on 100% homology for the immunogen sequence. Cross reactivity with AP2A proteins from other sources is not known.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1-25
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of Human AP2A1 and AP2A2 proteins.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
TFAP2A / AP-2
StandardGeneSymbol :
TFAP2A
Reactivity :
Chicken, Mouse, Dog, Rat, Chimpanzee, Orangutan, Human
Usage :
ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:500 - 1:2000)
ShortWebDescription :
AP-2 antibody LS-B427 is an unconjugated goat polyclonal antibody to AP-2 (TFAP2A) (aa1-25) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B427 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B427 was determined to be 2.5 ug/ml.
Synonyms :
TFAP2A, Activator protein 2, AP-2, AP-2a, AP2TF, AP-2alpha, BOFS, TFAP2, Transcription factor AP-2, AP-2 transcription factor, AP2-alpha
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.