product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ SPP1 / Osteopontin Antibody LS-B425
catalog :
LS-B425
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, dogs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
|
image
image 1 :
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
product information
AntibodyID :
31384
AntibodyName :
LS-B425
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
PathPlus™ SPP1 / Osteopontin Antibody LS-B425
Specificity :
The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
PurificationDesc :
Antiserum
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Gene :
SPP1 / Osteopontin
StandardGeneSymbol :
SPP1
Reactivity :
Mouse, Dog, Rat, Pig, Human
Usage :
ELISA (1:5000 - 1:20000), IHC, IHC-P (1:500), IP (1:100), WB (1:500 - 1:2000)
ShortWebDescription :
Osteopontin antibody LS-B425 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B425 was determined to be 1:500.
Synonyms :
SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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