product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ THBS1 / Thrombospondin-1 Antibody (aa19-32) LS-B4155
catalog :
LS-B4155
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Schips T, Vanhoutte D, Vo A, Correll R, Brody M, Khalil H, et al. Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. Nat Commun. 2019;10:76 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B4155 image 1
Anti-THBS1 / Thrombospondin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
image 2 :
LifeSpan Biosciences LS-B4155 image 2
THBS1 / Thrombospondin-1 antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
image 3 :
LifeSpan Biosciences LS-B4155 image 3
THBS1 / Thrombospondin-1 antibody (1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
product information
AntibodyID :
117007
AntibodyName :
LS-B4155
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ THBS1 / Thrombospondin-1 Antibody (aa19-32) LS-B4155
Specificity :
Human THBS1 / Thrombospondin.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa19-32
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence NRIPESGGDNSVFD-C, from the N-Terminus of protein sequence according to NP_003237.2.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
THBS1 / Thrombospondin-1
BlockingPeptide :
LS-E46706
StandardGeneSymbol :
THBS1
Reactivity :
Mouse, Bovine, Pig, Horse, Human, Monkey
Usage :
IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml)
ShortWebDescription :
Thrombospondin-1 antibody LS-B4155 is an unconjugated goat polyclonal antibody to Thrombospondin-1 (THBS1) (aa19-32) from human. It is reactive with human, mouse, bovine and other species. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B4155 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4155 was determined to be 3.75 ug/ml.
Synonyms :
THBS1, Thrombospondin-1, Thrombospondin-1p180, TSP, TSP1, THBS-1, THBS, Thrombospondin 1, TSP-1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.