product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TRPM2 Antibody (aa1139-1150) LS-B4122
catalog :
LS-B4122
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human
  • immunocytochemistry; human; fig 5
  • immunohistochemistry; human
  • western blot; human; 1:1000; fig 5
Tseng H, Vong C, Kwan Y, Lee S, Hoi M. TRPM2 regulates TXNIP-mediated NLRP3 inflammasome activation via interaction with p47 phox under high glucose in human monocytic cells. Sci Rep. 2016;6:35016 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B4122 image 1
Anti-TRPM2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
image 2 :
LifeSpan Biosciences LS-B4122 image 2
TRPM2 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
image 3 :
LifeSpan Biosciences LS-B4122 image 3
TRPM2 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
product information
AntibodyID :
116974
AntibodyName :
LS-B4122
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ TRPM2 Antibody (aa1139-1150) LS-B4122
Specificity :
Human TRPM2.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1139-1150
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-QQKQRPEQKIED, from the internal region of the protein sequence according to NP_003298.1NP_001307279.1NP_001307280.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
TRPM2
BlockingPeptide :
LS-E47769
StandardGeneSymbol :
TRPM2
gene family :
Ion Channel
Subfamily :
Calcium channel - TRP
Reactivity :
Human, Monkey
Usage :
IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000)
ShortWebDescription :
TRPM2 antibody LS-B4122 is an unconjugated goat polyclonal antibody to TRPM2 (aa1139-1150) from human. It is reactive with human and monkey. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B4122 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4122 was determined to be 3.75 ug/ml.
Synonyms :
TRPM2, EREG1, LTrpC-2, LTRPC2, NUDT9L1, KNP3, NUDT9H, Trrp7
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.