IHC-plus™ MCAM / CD146 Antibody (clone P1H12) LS-B4050 | LifeSpan Biosciences LS-B4050 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ MCAM / CD146 Antibody (clone P1H12) LS-B4050

catalog :

LS-B4050

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P1H12

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human	Yazawa E, Geddes Sweeney J, Cedeno Laurent F, Walley K, Barthel S, Opperman M, et al. Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential. J Invest Dermatol. 2015;135:1849-1862 pubmed publisher

image

image 1 :

Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

product information

AntibodyID :

116062

AntibodyName :

LS-B4050

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ MCAM / CD146 Antibody (clone P1H12) LS-B4050

ClonalityDesc :

Monoclonal

CloneName :

P1H12

AntibodyModification :

Unconjugated

PresentationDesc :

Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.

ImmunogenDesc :

Human umbilical vein cells

PurificationDesc :

Affinity purified

RecommendedStorageDesc :

Store at 4°C, avoid freeze-thaw cycles.

IsotypeName :

IgG1

Gene :

MCAM / CD146

StandardGeneSymbol :

MCAM

gene family :

Immunoglobulin

Reactivity :

Human

Usage :

Flo, IHC, IHC-Fr (1 - 10 µg/ml), IHC-P (20 µg/ml), IP, WB (1 - 5 µg/ml)

ShortWebDescription :

CD146 antibody LS-B4050 is an unconjugated mouse monoclonal antibody to human CD146 (MCAM). Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of HUVEC cell line. This can be used at 0.25 ul per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Synonyms :

MCAM, CD146, CD146 antigen, Gicerin, Melanoma adhesion molecule, MUC18, p1h12

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.