product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ NOTCH2 Antibody (aa2390-2415) LS-B399
catalog :
LS-B399
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 1
image
image 1 :
Anti-NOTCH2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
product information
AntibodyID :
31358
AntibodyName :
LS-B399
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
PathPlus™ NOTCH2 Antibody (aa2390-2415) LS-B399
Specificity :
This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa2390-2415
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.
PurificationDesc :
Antiserum
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Gene :
NOTCH2
StandardGeneSymbol :
NOTCH2
Reactivity :
Dog, Human, Chimpanzee
Usage :
ELISA (1:30000 - 1:90000), IHC, IHC-P (1:500), WB (1:400 - 1:2000)
ShortWebDescription :
NOTCH2 antibody LS-B399 is an unconjugated rabbit polyclonal antibody to NOTCH2 (aa2390-2415) from human. It is reactive with human, chimpanzee and dog. Validated for ELISA, IHC and WB. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B399 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B399 was determined to be 1:500.
Synonyms :
NOTCH2, AGS2, HN2, Notch (Drosophila) homolog 2, Notch homolog 2 (Drosophila), Notch 2, Notch homolog 2, HJCYS
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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