product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NODAL Antibody (aa242-253) LS-B3955
catalog :
LS-B3955
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse
  • immunohistochemistry; human; 1:100; fig s1
Margaryan N, Gilgur A, Seftor E, Purnell C, Arva N, Gosain A, et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci. 2016;17:418 pubmed publisher
HENDRIX M, Kandela I, Mazar A, Seftor E, SEFTOR R, Margaryan N, et al. Targeting melanoma with front-line therapy does not abrogate Nodal-expressing tumor cells. Lab Invest. 2017;97:176-186 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3955 image 1
Anti-NODAL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
product information
AntibodyID :
114870
AntibodyName :
LS-B3955
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ NODAL Antibody (aa242-253) LS-B3955
Specificity :
Human NODAL.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa242-253
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence DRSQLCRKVKFQ, from the internal region of the protein sequence according to NP_060525.2.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
NODAL
BlockingPeptide :
LS-E46651
StandardGeneSymbol :
NODAL
Reactivity :
Dog, Bovine, Pig, Horse, Bat, Human, Monkey
Usage :
IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:64000), WB (0.01 - 0.1 µg/ml)
ShortWebDescription :
NODAL antibody LS-B3955 is an unconjugated goat polyclonal antibody to NODAL (aa242-253) from human. It is reactive with human, bat, bovine and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B3955 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3955 was determined to be 3.75 ug/ml.
Synonyms :
NODAL, HTX5, Nodal homolog, Nodal, mouse, homolog, Nodal homolog (mouse)
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.