product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PTCH1 / Patched 1 Antibody (aa1431-1442) LS-B3939
catalog :
LS-B3939
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • ELISA; human
  • western blot; human; 1:1000; fig 1
Klein C, Zwick A, Kissel S, Forster C, Pfeifer D, Follo M, et al. Ptch2 loss drives myeloproliferation and myeloproliferative neoplasm progression. J Exp Med. 2016;213:273-90 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3939 image 1
Anti-PTCH1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
image 2 :
LifeSpan Biosciences LS-B3939 image 2
PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
product information
AntibodyID :
114854
AntibodyName :
LS-B3939
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ PTCH1 / Patched 1 Antibody (aa1431-1442) LS-B3939
Specificity :
Human PTCH1. This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1431-1442
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence ELQDVECEERPR, from the C-Terminus of protein sequence according to NP_000255.1NP_001077072.1NP_001077071.1NP_001077073.1NP_001077074.1NP_001077075.1NP_001077076.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
PTCH1 / Patched 1
BlockingPeptide :
LS-E25995
StandardGeneSymbol :
PTCH1
Reactivity :
Dog, Horse, Bat, Human, Monkey
Usage :
IF (5 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:8000)
ShortWebDescription :
Patched 1 antibody LS-B3939 is an unconjugated goat polyclonal antibody to Patched 1 (PTCH1) (aa1431-1442) from human. It is reactive with human, bat, dog and other species. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Synonyms :
PTCH1, NBCCS, Patched 1, Patched homolog 1, Protein patched homolog 1, PTC1, PTCH, PTCH protein +12b, PTCH protein -10, PTCH protein -3,4,5, Patched, Patched protein homolog 1, BCNS, HPE7, Patched homolog, PTC, PTCH protein +4, PTCH11
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.