product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ ACTA2 / Smooth Muscle Actin Antibody (aa1-50) LS-B3933
catalog :
LS-B3933
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; human
Westendorp B, Büller N, Karpus O, van Dop W, Koster J, Versteeg R, et al. Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response. Cell Mol Gastroenterol Hepatol. 2018;5:67-82.e1 pubmed publisher
Graupp M, Rinner B, Frisch M, Weiss G, Fuchs J, Sundl M, et al. Towards an in vitro fibrogenesis model of human vocal fold scarring. Eur Arch Otorhinolaryngol. 2018;275:1211-1218 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3933 image 1
ACTA2 / Smooth Muscle Actin antibody (0.5µg/ml) staining of Human (A) and (2ug/ml) Rat (B) Duodenum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
image 2 :
LifeSpan Biosciences LS-B3933 image 2
Anti-ACTA2 antibody IHC of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
image 3 :
LifeSpan Biosciences LS-B3933 image 3
ACTA2 / Smooth Muscle Actin antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
product information
AntibodyID :
114848
AntibodyName :
LS-B3933
TargetSpecies :
Human
Host Species :
Goat
Product Name :
PathPlus™ ACTA2 / Smooth Muscle Actin Antibody (aa1-50) LS-B3933
Specificity :
Human ACTA2. Variants NP_001604.1 and NP_001135417.1 encode the same protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1-50
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A synthetic peptide corresponding to the N-Terminus of human Alpha-Smooth Muscle Actin (ACTA2).
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
ACTA2 / Smooth Muscle Actin
BlockingPeptide :
LS-E25755
StandardGeneSymbol :
ACTA2
Reactivity :
Chicken, Bovine, Rat, Hamster, Pig, Horse, Bat, Rabbit, Mouse, Dog, Human, Monkey
Usage :
IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:64000), WB (0.1 - 1 µg/ml)
ShortWebDescription :
Smooth Muscle Actin antibody LS-B3933 is an unconjugated goat polyclonal antibody to Smooth Muscle Actin (ACTA2) (aa1-50) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Cited in 2 publications.
Synonyms :
ACTA2, ACTVS, Alpha-actin-2, Alpha-cardiac actin, AAT6, Actin, aortic smooth muscle, ACTSA, Smooth Muscle Actin, MYMY5, SMA
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.