product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ WNT5A Antibody (clone 6F2) LS-B3859
catalog :
LS-B3859
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6F2
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human
Prgomet Z, Andersson T, Lindberg P. Optimization, validation, and identification of two reliable antibodies for immunodetection of WNT5A. Biotech Histochem. 2017;92:46-58 pubmed publisher
Maekawa T, Kulwattanaporn P, Hosur K, Domon H, Oda M, Terao Y, et al. Differential Expression and Roles of Secreted Frizzled-Related Protein 5 and the Wingless Homolog Wnt5a in Periodontitis. J Dent Res. 2017;96:571-577 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3859 image 1
Anti-WNT5A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
product information
AntibodyID :
111878
AntibodyName :
LS-B3859
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ WNT5A Antibody (clone 6F2) LS-B3859
Specificity :
Human
ClonalityDesc :
Monoclonal
CloneName :
6F2
AntibodyModification :
Unconjugated
PresentationDesc :
Ascitic fluid, 0.03% sodium azide
ImmunogenDesc :
Purified recombinant fragment of WNT5A expressed in E. Coli.
PurificationDesc :
Ascites
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C.
IsotypeName :
IgG1
Gene :
WNT5A
StandardGeneSymbol :
WNT5A
gene family :
WNT
Reactivity :
Human
Usage :
ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:400), WB (1:100 - 1:500)
ShortWebDescription :
WNT5A antibody LS-B3859 is an unconjugated mouse monoclonal antibody to human WNT5A. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B3859 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3859 was determined to be 1:200.
Synonyms :
WNT5A, HWNT5A, Protein Wnt-5a, WNT-5A protein
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.