product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ HSPB1 / HSP27 Antibody (clone G3.1) LS-B383
catalog :
LS-B383
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
G3.1
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 3
Reference
Edwards D, Adams D, McGuire W. Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7). Breast Cancer Res Treat. 1981;1:209-23 pubmed
Moretti Rojas I, Fuqua S, Montgomery R, McGuire W. A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Breast Cancer Res Treat. 1988;11:155-63 pubmed
McGuire S, Fuqua S, Naylor S, Helin Davis D, McGuire W. Chromosomal assignments of human 27-kDa heat shock protein gene family. Somat Cell Mol Genet. 1989;15:167-71 pubmed
image
image 1 :
LifeSpan Biosciences LS-B383 image 1
Anti-HSPB1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
31342
AntibodyName :
LS-B383
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PathPlus™ HSPB1 / HSP27 Antibody (clone G3.1) LS-B383
Specificity :
Reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control. Cross reactivity with hsp27 from other mammalian sources is likely. No cross reactivity occurs with HSP70, HSP90 or HSP104.
ClonalityDesc :
Monoclonal
CloneName :
G3.1
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
HSPB1 / HSP27
StandardGeneSymbol :
HSPB1
Reactivity :
Human
Usage :
ELISA (1:10000 - 1:50000), IHC, IHC-P (1.25 µg/ml), WB (1:500 - 1:1000)
ShortWebDescription :
HSP27 antibody LS-B383 is an unconjugated mouse monoclonal antibody to human HSP27 (HSPB1). Validated for ELISA, IHC and WB. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B383 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B383 was determined to be 1.25 ug/ml.
Synonyms :
HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.