This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MLC2 / MYL9 Antibody LS-B38
catalog :
LS-B38
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
27078
AntibodyName :
LS-B38
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ MLC2 / MYL9 Antibody LS-B38
Specificity :
The antibody detects both unphosphorylated and monophosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity is expected with myosin light chain from human, mouse and rat sources. Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids aa 10-35 of human myosin light chain protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
IsotypeName :
IgG
Gene :
MLC2 / MYL9
StandardGeneSymbol :
MYL9
gene family :
Protein Kinase Non Catalytic
Reactivity :
Mouse, Rat, Human
Usage :
ELISA (1:10000 - 1:70000), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:500 - 1:2000)
ShortWebDescription :
MYL9 antibody LS-B38 is an unconjugated rabbit polyclonal antibody to MYL9 (MLC2) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B38 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B38 was determined to be 2.5 ug/ml.
Synonyms :
MYL9, 20 kDa myosin light chain, LC20, MLC-2C, MLC2, Myosin RLC, MRLC1, MYRL2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.