product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CD209 / DC-SIGN Antibody (clone h209) LS-B3782
catalog :
LS-B3782
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
h209
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Chan S, Filézac de L Etang A, Rangell L, Caplazi P, Lowe J, Romeo V. A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples. PLoS ONE. 2018;13:e0207619 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3782 image 1
Anti-CD209 / DC-SIGN antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
product information
AntibodyID :
111801
AntibodyName :
LS-B3782
TargetSpecies :
Human
Host Species :
Rat
Product Name :
IHC-plus™ CD209 / DC-SIGN Antibody (clone h209) LS-B3782
ClonalityDesc :
Monoclonal
CloneName :
h209
AntibodyModification :
Unconjugated
PresentationDesc :
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
ImmunogenDesc :
Human CD209
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C.
IsotypeName :
IgG2a,k
Gene :
CD209 / DC-SIGN
StandardGeneSymbol :
CD209
Reactivity :
Human
Usage :
Flo, IHC, IHC-P (20 µg/ml), IP
ShortWebDescription :
DC-SIGN antibody LS-B3782 is an unconjugated rat monoclonal antibody to human DC-SIGN (CD209). Validated for Flow, IHC and IP. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B3782 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3782 was determined to be 20 ug/ml. Tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Synonyms :
CD209, CD209 antigen, CD209 molecule, CDSIGN, CLEC4L, DC-SIGN, HIV gpl20-binding protein, DC-SIGN1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.