This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ 4E-BP2 / EIF4EBP2 Antibody (aa99-120) LS-B3749
catalog :
LS-B3749
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
acetylated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
111768
AntibodyName :
LS-B3749
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ 4E-BP2 / EIF4EBP2 Antibody (aa99-120) LS-B3749
Specificity :
Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa99-120
PresentationDesc :
25 mM Sodium Borate, BBS, pH 8.4, 75 mM NaCl, 5 mM EDTA, 100 mM Boric acid
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Human PHAS-II synthetic peptide (aa 99-120) was synthesized and the peptide coupled to KLH. This sequence is 96% homologous to mouse PHAS-11 over these residues
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Gene :
4E-BP2 / EIF4EBP2
StandardGeneSymbol :
EIF4EBP2
Reactivity :
Mouse, Dog, Bovine, Rat, Pig, Human
Usage :
IHC, IHC-P (10 µg/ml), WB (4 µg/ml)
ShortWebDescription :
EIF4EBP2 antibody LS-B3749 is an unconjugated rabbit polyclonal antibody to EIF4EBP2 (4E-BP2) (aa99-120) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3749 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3749 was determined to be 10 ug/ml.
Synonyms :
EIF4EBP2, 4E-BP2, 4EBP2, EIF4E-binding protein 2, PHASII
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.