IHC-plus™ CAMK2 / CAMKII Antibody LS-B3743 | LifeSpan Biosciences LS-B3743 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ CAMK2 / CAMKII Antibody LS-B3743

catalog :

LS-B3743

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1a	Prasad A, Ketsawatsomkron P, Nuno D, Koval O, Dibbern M, Venema A, et al. Role of CaMKII in Ang-II-dependent small artery remodeling. Vascul Pharmacol. 2016;87:172-179 pubmed publisher

image

image 1 :

image 2 :

Anti-CAMK2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

product information

AntibodyID :

111762

AntibodyName :

LS-B3743

TargetSpecies :

Rat

Host Species :

Rabbit

Product Name :

IHC-plus™ CAMK2 / CAMKII Antibody LS-B3743

Specificity :

Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

PresentationDesc :

25 mM Sodium Borate, BBS, pH 8.2, 75 mM NaCl, 5 mM EDTA, 100 mM Boric acid

ImmunogenType :

Synthetic peptide - KLH conjugated

ImmunogenDesc :

A 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (aa 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%); Orangutan (93%).

PurificationDesc :

Immunoaffinity purified

RecommendedStorageDesc :

Store at -20°C.

Gene :

CAMK2 / CAMKII

StandardGeneSymbol :

CAMK2

Reactivity :

Xenopus, Chicken, Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Bovine, Rat, Human, Monkey

Usage :

IHC, IHC-P (10 µg/ml), WB (1:1000)

ShortWebDescription :

CAMKII antibody LS-B3743 is an unconjugated rabbit polyclonal antibody to CAMKII (CAMK2) from rat. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B3743 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3743 was determined to be 10 ug/ml.

Synonyms :

, CAMK2

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.