product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CRYAB / Alpha B Crystallin Antibody LS-B3696
catalog :
LS-B3696
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, pigs
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; pigs
Gedik N, Kruger M, Thielmann M, Kottenberg E, Skyschally A, Frey U, et al. Proteomics/phosphoproteomics of left ventricular biopsies from patients with surgical coronary revascularization and pigs with coronary occlusion/reperfusion: remote ischemic preconditioning. Sci Rep. 2017;7:7629 pubmed publisher
Mathys H, Davila Velderrain J, Peng Z, Gao F, Mohammadi S, Young J, et al. Single-cell transcriptomic analysis of Alzheimer's disease. Nature. 2019;570:332-337 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3696 image 1
Anti-CRYAB / Alpha B Crystallin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
image 2 :
LifeSpan Biosciences LS-B3696 image 2
Western blot of SPA-223: Lane 1: MWM, Lane 2: SPP-225 Lane3: SPP-226, Lane4: SPP-227, Lane 5: SPP-235.
product information
AntibodyID :
111053
AntibodyName :
LS-B3696
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ CRYAB / Alpha B Crystallin Antibody LS-B3696
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide, 50% Glycerol
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
Synthetic peptide derived from sequence near the C-Terminus of human alphaB-Crystallin, conjugated to KLH; sequence identical to rat, rabbit, and bovine.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Gene :
CRYAB / Alpha B Crystallin
StandardGeneSymbol :
CRYAB
Reactivity :
Mouse, Bovine, Rat, Pig, Human
Usage :
IHC, IHC-P (10 µg/ml), WB (1:1000)
ShortWebDescription :
Alpha B Crystallin antibody LS-B3696 is an unconjugated rabbit polyclonal antibody to Alpha B Crystallin (CRYAB) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B3696 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3696 was determined to be 10 ug/ml
Synonyms :
CRYAB, Alpha-crystallin B chain, CRYA2, Crystallin, alpha B, CTPP2, Heat shock protein beta-5, HSPB5, Alpha B Crystallin, Alpha(B)-crystallin, Rosenthal fiber component, Heat-shock 20 kD like-protein
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.