product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SELP / P-Selectin / CD62P Antibody (clone Psel.KO.2.5) LS-B3656
catalog :
LS-B3656
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Psel.KO.2.5
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human
Nolo R, Herbrich S, Rao A, Zweidler Mckay P, Kannan S, Gopalakrishnan V. Targeting P-selectin blocks neuroblastoma growth. Oncotarget. 2017;8:86657-86670 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3656 image 1
Anti-CD62P antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
110788
AntibodyName :
LS-B3656
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ SELP / P-Selectin / CD62P Antibody (clone Psel.KO.2.5) LS-B3656
Specificity :
Recognizes the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
ClonalityDesc :
Monoclonal
CloneName :
Psel.KO.2.5
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Cells
ImmunogenDesc :
CD62P transfected 300.19 cells
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
SELP / P-Selectin / CD62P
StandardGeneSymbol :
SELP
Reactivity :
Human
Usage :
Flo (1:1 - 1:50), IHC, IHC-P (10 µg/ml), IP
ShortWebDescription :
CD62P antibody LS-B3656 is an unconjugated mouse monoclonal antibody to human CD62P (SELP / P-Selectin). Validated for Flow, IHC and IP. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B3656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3656 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Synonyms :
SELP, CD62P, CD62, GMP-140, GMRP, Granule membrane protein 140, LECAM3, P-selectin, PSEL, CD62P antigen, GMP140, Granulocyte membrane protein, GRMP, PADGEM
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.