This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Collagen IV Antibody LS-B3654
catalog :
LS-B3654
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
110786
AntibodyName :
LS-B3654
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ Collagen IV Antibody LS-B3654
Specificity :
Human Type IV collagen. Cross-reactivity: Human collagen type I: 1% Human collagen type II: <0.5%, Human collagen type III: <0.5%, Human collagen type IV: 100%, Human collagen type V: 7%, Human fibronectin: <0.5%, Human albumin: <0.5%, Human immunoglobulin <0.5%.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, mannitol, dextran, no preservatives.
ImmunogenType :
Purified protein
ImmunogenDesc :
Human placental collagen type IV.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: 4°C Long term: Aliquot and store at -20°C.
IsotypeName :
IgG
Gene :
Collagen IV
Reactivity :
Human
Usage :
ELISA (1:3000), IF, IHC, IHC-Fr, IHC-P (1:100)
UsageNotRecommended :
WB
ShortWebDescription :
Collagen IV antibody LS-B3654 is an unconjugated rabbit polyclonal antibody to human Collagen IV. Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3654 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40. fresh frozen or acetone fixed specimens. Dot Blotting: 1:300. not recommended for Western Blots.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.