This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ S100A9 / MRP14 Antibody (aa67-79) LS-B3613
catalog :
LS-B3613
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
110163
AntibodyName :
LS-B3613
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ S100A9 / MRP14 Antibody (aa67-79) LS-B3613
Specificity :
Human S100A9.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa67-79
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-DTNADKQLSFEEF, from the internal region of the protein sequence according to NP_002956.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
S100A9 / MRP14
BlockingPeptide :
LS-E26976
StandardGeneSymbol :
S100A9
Reactivity :
Human
Usage :
IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:8000), WB (0.3 - 1 µg/ml)
ShortWebDescription :
MRP14 antibody LS-B3613 is an unconjugated goat polyclonal antibody to human MRP14 (S100A9) (aa67-79). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3613 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3613 was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 16kD band observed in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kD according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kD) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16;280(50):41521-9.; PMID: 16216873). Recommended concentration: 0.3-1 ug/ml.
Synonyms :
S100A9, 60B8AG, Calgranulin B, CFAG, CAGB, Calgranulin-B, Calprotectin L1H subunit, CGLB, L1Ag, MRP14, NIF, p14, Protein S100-A9, LIAG, MAC387, MRP-14
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.