product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ GRIA4 / GLUR4 Antibody (aa279-292) LS-B3606
catalog :
LS-B3606
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Gok Yurtseven D, Serter Kocoglu S, Minbay Z, Eyigor O. Immunohistochemical Evidence for Glutamatergic Regulation of Nesfatin-1 Neurons in the Rat Hypothalamus. Brain Sci. 2020;10: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3606 image 1
Antibody (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
image 2 :
LifeSpan Biosciences LS-B3606 image 2
Anti-GRIA4 / GLUR4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
110156
AntibodyName :
LS-B3606
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ GRIA4 / GLUR4 Antibody (aa279-292) LS-B3606
Specificity :
Human GRIA4 / GLUR4. This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa279-292
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3NP_001070711.2NP_001070712.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
GRIA4 / GLUR4
BlockingPeptide :
LS-E26704
StandardGeneSymbol :
GRIA4
gene family :
Ion Channel
Subfamily :
Glutamate receptor - ionotropic (NMDA receptor)
Reactivity :
Chicken, Bovine, Rabbit, Mouse, Dog, Rat, Horse, Bat, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 2 µg/ml)
ShortWebDescription :
GLUR4 antibody LS-B3606 is an unconjugated goat polyclonal antibody to GLUR4 (GRIA4) (aa279-292) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B3606 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3606 was determined to be 5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 90 kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kD according to NP_001070711.1). Recommended concentration: 0.3-1 ug/ml.
Synonyms :
GRIA4, GluA4, GluR-4, GLUR4, GLUR4C, Glutamate receptor type d, GluR-D, GLURD, Glutamate receptor 4
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.