product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Cullin 4A / CUL4A Antibody (aa1-25) LS-B360
catalog :
LS-B360
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Meerang M, Kreienbühl J, Orlowski V, Müller S, Kirschner M, Opitz I. Importance of Cullin4 Ubiquitin Ligase in Malignant Pleural Mesothelioma. Cancers (Basel). 2020;12: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B360 image 1
Anti-CUL4A / Cullin 4A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
product information
AntibodyID :
31319
AntibodyName :
LS-B360
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ Cullin 4A / CUL4A Antibody (aa1-25) LS-B360
Specificity :
Reacts with human Cullin 4A. Cross reactivity is expected against mouse Cul4A based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1-25
PresentationDesc :
Antiserum, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of Human Cul4A coupled to KLH.
PurificationDesc :
Delipidated and defibrinated followed by sterile filtration
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Gene :
Cullin 4A / CUL4A
StandardGeneSymbol :
CUL4A
Reactivity :
Human
Usage :
ELISA (1:2000 - 1:10000), IHC, IHC-P (1:500), IP (1:500), WB (1:500 - 1:1000)
ShortWebDescription :
CUL4A antibody LS-B360 is an unconjugated rabbit polyclonal antibody to human CUL4A (Cullin 4A) (aa1-25). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B360 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B360 was determined to be 1:500.
Synonyms :
CUL4A, CUL-4A, Cullin-4A, Cullin 4A
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.