This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ BRCC3 / BRCC36 Antibody (clone AT3B1) LS-B3588
catalog :
LS-B3588
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AT3B1

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
109474
AntibodyName :
LS-B3588
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ BRCC3 / BRCC36 Antibody (clone AT3B1) LS-B3588
Specificity :
BRCC36 (1-316aa)
ClonalityDesc :
Monoclonal
CloneName :
AT3B1
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 0.1% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant BRCC36 (1-316aa) purified from E. coli
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
BRCC3 / BRCC36
StandardGeneSymbol :
BRCC3
Reactivity :
Human
Usage :
ELISA, IHC, IHC-P (5 µg/ml), WB (1:1000 - 1:2000)
ShortWebDescription :
BRCC36 antibody LS-B3588 is an unconjugated mouse monoclonal antibody to human BRCC36 (BRCC3). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3588 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3588 was determined to be 5 ug/ml. Western Blot: Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Synonyms :
BRCC3, C6.1A, BRCC36, BRISC complex subunit BRCC36, BRCA1-A complex subunit BRCC36, CXorf53, RP11-143H17.2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.