product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ HK2 / Hexokinase 2 Antibody (aa74-123) LS-B3571
catalog :
LS-B3571
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Gong L, Zhang Q, Pan X, Chen S, Yang L, Liu B, et al. p53 Protects Cells from Death at the Heatstroke Threshold Temperature. Cell Rep. 2019;29:3693-3707.e5 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3571 image 1
Anti-HK2 / Hexokinase 2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
109457
AntibodyName :
LS-B3571
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ HK2 / Hexokinase 2 Antibody (aa74-123) LS-B3571
Specificity :
Human HK2 / Hexokinase 2
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa74-123
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide located between aa74-123 of human HK2 (P52789, NP_000180). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Sablefish, Stickleback (92%); Orangutan, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish, Sea squirt (85%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
HK2 / Hexokinase 2
BlockingPeptide :
LS-E19535
StandardGeneSymbol :
HK2
gene family :
Non-protein Kinase
Subfamily :
Hexokinase
Reactivity :
Zebrafish, Pig, Horse, Bat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), WB
ShortWebDescription :
Hexokinase 2 antibody LS-B3571 is an unconjugated rabbit polyclonal antibody to Hexokinase 2 (HK2) (aa74-123) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B3571 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3571 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Synonyms :
HK2, Hexokinase II, Hexokinase type II, Hexokinase-2, muscle, HK II, HXK2, Hexokinase-2, Muscle form hexokinase, Hexokinase 2, HKII, Hxk 2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.