product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NR0B2 Antibody (aa110-159) LS-B3560
catalog :
LS-B3560
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human
Prestin K, Olbert M, Hussner J, Isenegger T, Gliesche D, Böttcher K, et al. Modulation of expression of the nuclear receptor NR0B2 (small heterodimer partner 1) and its impact on proliferation of renal carcinoma cells. Onco Targets Ther. 2016;9:4867-78 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3560 image 1
Anti-NR0B2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
109446
AntibodyName :
LS-B3560
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ NR0B2 Antibody (aa110-159) LS-B3560
Specificity :
Human NR0B2
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa110-159
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide located between aa110-159 of human NR0B2 (Q15466, NP_068804). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin, Dog, Rabbit (100%); Baboon, Galago (92%); Horse (91%); Elephant, Guinea pig (85%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
NR0B2
BlockingPeptide :
LS-E16665
StandardGeneSymbol :
NR0B2
gene family :
NHR
Subfamily :
NR0 Knirps-like
Reactivity :
Rabbit, Dog, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), WB
ShortWebDescription :
NR0B2 antibody LS-B3560 is an unconjugated rabbit polyclonal antibody to NR0B2 (aa110-159) from human. It is reactive with human, dog, monkey and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B3560 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3560 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Synonyms :
NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.