product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-SOX4
catalog :
LS-B3520
quantity :
price :
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
immunohistochemistry - paraffin section, western blot, ELISA, immunohistochemistry
publications citing this reagent :
  1. Lai Y, Cheng J, Cheng D, Feasel M, Beste K, Peng J, et al. SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells. BMC Cell Biol. 2011;12:50 pubmed publisher
more info or order :
LifeSpan Biosciences product webpage
image
image 1 :
LifeSpan Biosciences LS-B3520 image 1
Human Breast (formalin-fixed, paraffin-embedded) stained with SOX4 antibody LS-B3520 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
image 2 :
LifeSpan Biosciences LS-B3520 image 2
Human Heart (formalin-fixed, paraffin-embedded) stained with SOX4 antibody LS-B3520 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
image 3 :
LifeSpan Biosciences LS-B3520 image 3
Human Testis (formalin-fixed, paraffin-embedded) stained with SOX4 antibody LS-B3520 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
product information
?AntibodyID :
107685
AntibodyName :
LS-B3520
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
Anti-SOX4
Specificity :
Human SOX4
ClonalityDesc :
Polyclonal
AntigenModification :
aa38-87
PresentationDesc :
Lyophilized from PBS with 2% sucrose
ImmunogenDesc :
Synthetic peptide located between aa38-87 of human SOX4 (Q06945, NP_003098). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Lizard, Xenopus, Seabass, Medaka, Pufferfish, Zebrafish, Drosophila, Beetle (100%); Horse, Zebra finch, Salmon (92%); Panda (90%); Trout (85%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
ResuspensionLiquidDesc :
Distilled water
IsotypeName :
IgG
Gene :
SOX4
StandardGeneSymbol :
SOX4
gene family :
Transcription factor
Reactivity :
Gibbon, Bovine, Chimpanzee, Chicken, Dog, Drosophila, Xenopus, Guinea pig, Human, Monkey, Mouse, Pig, Rabbit, Rat, Zebrafish
Usage :
IHC-P (5 µg/ml), WB
UsageText :
Immunohistochemistry: LS-B3520 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3520 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Synonyms :
SOX4, EVI16, SRY-related HMG-box gene 4, Transcription factor SOX-4
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.