product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ATF4 Antibody (N-Terminus) LS-B3517
catalog :
LS-B3517
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Freundt J, Frommeyer G, Wötzel F, Huge A, Hoffmeier A, Martens S, et al. The Transcription Factor ATF4 Promotes Expression of Cell Stress Genes and Cardiomyocyte Death in a Cellular Model of Atrial Fibrillation. Biomed Res Int. 2018;2018:3694362 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3517 image 1
Anti-ATF4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
107682
AntibodyName :
LS-B3517
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
IHC-plus™ ATF4 Antibody (N-Terminus) LS-B3517
Specificity :
Mouse ATF4
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from N-Terminus of mouse ATF4 (Q61328, NP_033846). Percent identity by BLAST analysis: Mouse (100%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
ATF4
BlockingPeptide :
LS-E13279
StandardGeneSymbol :
ATF4
gene family :
Transcription factor
Reactivity :
Mouse, Human
Usage :
IHC, IHC-P (5 µg/ml), WB
ShortWebDescription :
ATF4 antibody LS-B3517 is an unconjugated rabbit polyclonal antibody to ATF4 (N-Terminus) from mouse. It is reactive with human and mouse. Validated for IHC and WB. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B3517 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3517 was determined to be 5 ug/ml.
Synonyms :
ATF4, CREB-2, CREB2, TXREB, DNA-binding protein TAXREB67, TAXREB67
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.