product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ IAPP / Amylin Antibody (clone R10/99, Supernatant) LS-B35
catalog :
LS-B35
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
R10/99
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Sormanni P, Aprile F, Vendruscolo M. Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins. Proc Natl Acad Sci U S A. 2015;112:9902-7 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B35 image 1
Anti-Amylin antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
27075
AntibodyName :
LS-B35
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ IAPP / Amylin Antibody (clone R10/99, Supernatant) LS-B35
Specificity :
Recognizes the islet amyloid polypeptide (amylin). Is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.
ClonalityDesc :
Monoclonal
CloneName :
R10/99
AntibodyModification :
Supernatant Unconjugated
PresentationDesc :
0.1M Tris, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
CATQRLANFLV coupled to tuberculin.
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
IAPP / Amylin
StandardGeneSymbol :
IAPP
Reactivity :
Human
Usage :
IF, IHC, IHC-Fr, IHC-P (1:100)
ShortWebDescription :
Amylin antibody LS-B35 is an unconjugated mouse monoclonal antibody to human Amylin (IAPP). Validated for IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B35 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B35 was determined to be 1:100.
Synonyms :
IAPP, Diabetes-associated peptide, Islet amyloid polypeptide, AMYLIN, IAP, Insulinoma amyloid peptide
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.