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product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ GATA3 Antibody (aa36-85) LS-B3482
catalog :
LS-B3482
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
107647
AntibodyName :
LS-B3482
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
PathPlus™ GATA3 Antibody (aa36-85) LS-B3482
Specificity :
Human GATA3
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa36-85
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide located between aa36-85 of human GATA3 (P23771, NP_002042). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Zebra finch, Chicken (100%); Bat, Guinea pig, Trout, Salmon (92%); Zebrafish (84%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
GATA3
BlockingPeptide :
LS-E11110
StandardGeneSymbol :
GATA3
gene family :
Transcription factor
Reactivity :
Chicken, Zebrafish, Pig, Horse, Sheep, Bovine, Rabbit, Mouse, Dog, Rat, Hamster, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
ShortWebDescription :
GATA3 antibody LS-B3482 is an unconjugated rabbit polyclonal antibody to GATA3 (aa36-85) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
UsageText :
Immunohistochemistry: LS-B3482 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3482 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Synonyms :
GATA3, GATA-binding factor 3, HDRS, GATA binding protein 3, GATA-binding protein 3, HDR
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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