product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PDK4 Antibody (aa382-410) LS-B3459
catalog :
LS-B3459
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human
Mazar J, Qi F, Lee B, Marchica J, Govindarajan S, Shelley J, et al. MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells. Mol Cell Biol. 2016;36:1090-108 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3459 image 1
Anti-PDK4 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
image 2 :
LifeSpan Biosciences LS-B3459 image 2
Fluorescent confocal image of HeLa cells stained with PDK4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated PDK4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.
image 3 :
LifeSpan Biosciences LS-B3459 image 3
The PDK4 Antibody is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.
product information
AntibodyID :
106703
AntibodyName :
LS-B3459
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ PDK4 Antibody (aa382-410) LS-B3459
Specificity :
This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa382-410
PresentationDesc :
PBS, 0.09% Sodium Azide
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Gene :
PDK4
BlockingPeptide :
LS-E8632
StandardGeneSymbol :
PDK4
gene family :
Protein Kinase
Subfamily :
PDK/BCKDK
Reactivity :
Mouse, Human
Usage :
IF (1:100), IHC, IHC-P (10 µg/ml), WB (1:1000)
ShortWebDescription :
PDK4 antibody LS-B3459 is an unconjugated rabbit polyclonal antibody to PDK4 (aa382-410) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
UsageText :
Immunohistochemistry: LS-B3459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3459 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use with mouse in IHC-P.
Synonyms :
PDK4, PDHK4
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.