product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CCND1 / Cyclin D1 Antibody (aa68-97) LS-B3452
catalog :
LS-B3452
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 1
image
image 1 :
Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
image 2 :
Western blot of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane). Cyclin D1(arrow) was detected using the purified antibody.
image 3 :
Confocal immunofluorescence of Cyclin D1 Antibody (S90) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
product information
AntibodyID :
106696
AntibodyName :
LS-B3452
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ CCND1 / Cyclin D1 Antibody (aa68-97) LS-B3452
Specificity :
This Cyclin D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human Cyclin D1.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa68-97
PresentationDesc :
PBS, 0.09% Sodium Azide
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Gene :
CCND1 / Cyclin D1
BlockingPeptide :
LS-E7012
StandardGeneSymbol :
CCND1
Reactivity :
Mouse, Human
Usage :
IF (1:10 - 1:50), IHC, IHC-P (10 µg/ml), WB (1:1000)
ShortWebDescription :
Cyclin D1 antibody LS-B3452 is an unconjugated rabbit polyclonal antibody to Cyclin D1 (CCND1) (aa68-97) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B3452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3452 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use in IHC with mouse samples.
Synonyms :
CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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