product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PECAM-1 / CD31 Antibody (clone 2F7B2) LS-B3446
catalog :
LS-B3446
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2F7B2
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human
Pestronk A, Schmidt R, Choksi R. Vascular pathology in dermatomyositis and anatomic relations to myopathology. Muscle Nerve. 2010;42:53-61 pubmed publisher
Collins K, Kang H, Matsche J, Klomp J, Rehman J, Malik A, et al. Septin2 mediates podosome maturation and endothelial cell invasion associated with angiogenesis. J Cell Biol. 2020;219: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3446 image 1
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
product information
AntibodyID :
106119
AntibodyName :
LS-B3446
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ PECAM-1 / CD31 Antibody (clone 2F7B2) LS-B3446
Specificity :
Human
ClonalityDesc :
Monoclonal
CloneName :
2F7B2
AntibodyModification :
Unconjugated
PresentationDesc :
Ascitic fluid, 0.03% sodium azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Purified recombinant fragment of human CD31 expressed in E. Coli.
PurificationDesc :
Ascites
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C.
IsotypeName :
IgG1
Gene :
PECAM-1 / CD31
StandardGeneSymbol :
PECAM1
Reactivity :
Human
Usage :
ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200), WB (1:100 - 1:500)
ShortWebDescription :
CD31 antibody LS-B3446 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B3446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3446 was determined to be 1:200.
Synonyms :
PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.